INCREASED CIRCULATING PLATELET-NEUTROPHIL COMPLEXES IN ADULT NEWLY DIAGNOSED NON-HODGKINS‘S LYMPHOMA PATIENTS
Keywords:
platelet-neutrophil complexes, aggressive and indolent Non-Hodgkin's lymphomaAbstract
Non-Hodgkin’s lymphoma (NHL) is a group of lymphoproliferative malignant disorders with heterogeneous histological, biological and clinical characteristics. Non-Hodgkin’s lymphoma is characterized by clonal abnormal proliferation and/or accumulation of B or T lymphocytes. Platelets and neutrophils interact during infection, inflammation and thrombosis and modulate each other’s functions.The formation of complexes between neutrophils and circulating platelets (platelet-neutrophil complexes - PNC) is a fundamental mechanism that is known but poorly studied in non-Hodgkin's lymphomas. Aims: We aimed to investigate the serum level of circulating platelet-neutrophil complexes in newly diagnosed non-Hodgkin's lymphoma (NHL) patients and their relationship with clinicopathological characteristics and prognostic parameters. Material and methods: A total 88 patients with pathologically verified NHL and 20 healthy controls were analyzed. 34 were with Diffuse large B-cell lymphoma (DLBCL), 9 with Mantel cell lymphoma( MCL), 4 with Peripheral T-cell lymphoma (PTCL), 15 with Marginal zone lymphoma (MZL), 10 with Follicular lymphoma( FL), 1 patient with Plasmoablastic lymphoma(PBL), 1 with Lymphoplasmacytic Lymphoma (LPL) and 14 with Small lymphocytic lymphoma (SLL). Serum levels of PNC were measured by flow cytometric analysis of peripheral blood. The results were statistically analyzed by dispersion, comparison and regression methods.
Results: PNC levels were significantly higher in patients with NHL compared to healthy controls (p<0.01). PNC levels were significantly higher in the group of patients with aggressive NHL compared with the group of patients with inolent NHL (respectively p= 0.05). There was a strong significant correlation between PNC and Platelett (р=0.032), PNC and Absolute Neutrophil Count (ANC) (р=0.015) and PNC and Hemoglobin (r = 0.463; p < 0.001). No correlation was found with leukocyte count, LDH, B symptoms, β2-микроглобулин and absolute lymphocyte count (ALC ). Conclusions: Elevated levels of PNC are associated with increased tumor growth and could be an independent negative prognostic factor in development of NHL.
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