USE OF SCREENING TESTS FOR CEFTIOFUR IN EVALUATION OF RAW MILK SAFETY

Authors

  • Dragan Ilievski University “St. Kliment Ohridski” – Bitola, Faculty of Biotechnical Sciences – Bitola, RN Macedonia
  • Biljana Trajkovska University “St. Kliment Ohridski” – Bitola, Faculty of Biotechnical Sciences – Bitola, RN Macedonia
  • Ljupche Kochoski University “St. Kliment Ohridski” – Bitola, Faculty of Biotechnical Sciences – Bitola, RN Macedonia

Keywords:

ceftiofur, antibiotics, milk, screening test, MRL

Abstract

In a modern dairy farming, it is getting more and more important cows to be treated with antibiotics with as less as possible withdrawal period for milk. Ceftiofur is one of such antibiotics that are recently widely used for cow’s treatment against different diseases including mastitis. That’s why a new generation of screening test kits sensitive for ceftiofur, like Milk Safe 3BTS, are available on the market. The aim of the study was to compare the results from different screening test on sensitivity of ceftiofur. In total 76 raw milk samples from sixteen treated cows were used during this study. Individual milk samples were tested using three commercial screening test kits for inhibitor residues: Milk Safe 3BTS, 4 Sensor and Eclipse Farm 3G.Results obtained during this study show different results. Milk Safe 3BTS gives the most positive results and higher sensitivity compare with the others. This is mainly due to different detection limits and only Milk Safe 3BTS has a specific sensitivity on ceftiofur residues. Generally, there are still residues of ceftiofur in milk sometimes even after 48 hours after application. Milk Safe 3BTS shows the better sensitivity results compares with the others. Milk Safe 3BTS can be used on a farm level as a screening test for detection of residues of ceftiofur

References

Alves, J. F., Paula, G. H., Silva, R. C., Leão, P. V., Leão, K. M., Nicolau, E. S., & Silva, M. A. (2019). Residues of

antibiotics in milk: persistence and quality interference. Canadian Journal of Animal Science, 100(1), 93-101.

Cristina, M., Melo, R.T., Mendonça, E.P., Coelho, L.R., & Rossi, D.A. (2010). Use of ceftiofur in dairy cows and their excretion in milk. PUBVET. 4: 931–937.

Durel, L., Gallina, G., & Pellet, T. (2019). Assessment of ceftiofur residues in cow milk using commercial screening test kits Veterinary Record Open 2019;0:e000329. doi:10.1136/ vetreco-2018-000329

European Agency for the Evaluation of Medicinal Products, Committee for Veterinary Medicinal Products,

Ceftiofur, Modification of MRLs for bovine species, Summary report 3, EMEA/MRL/835/02-FINAL, April 2002

Fejzic, N., Begagic, M., Šerić-Haračić, S., et al. (2014). Beta lactam antibiotics residues in cow's milk: comparison of efficacy of three screening tests used in Bosnia and Herzegovina. Bosn J Basic Med Sci 2014;14: 155–9 http://www.ncbi.nlm.nih.gov/pubmed/25172975. doi: https://doi.org/10.17305/bjbms.2014.3.109

Han, R., Li, S., Wang, J., Yu, Z., Wang, J., & Zheng, N. (2017). Elimination kinetics of ceftiofur hydrochloride in

milk after an 8-day extended intramammary administration in healthy and infected cows. PloS one, 12(11), e0187261. 3455-3461.

Jaglan, P. S., Yein, F. S., Hornish, R. E., Cox, B. L., Arnold, T. S., Roof, R. D., & Gilbertson, T. J. (1992).

Depletion of intramuscularly injected ceftiofur from the milk of dairy cattle. Journal of dairy science, 75(7), 1870-1876.

Lim, J. H., Park, B. K., Kim, M. S., Jang, B. S., Kim, D., & Yun, H. I. (2004). Residual determination of Ceftiofur

in Raw Bovine Milk by Liquid Chromatography-Electrospray Mass Spectrometry. Korean Journal of Veterinary Research, 44(3), 367-371.

Mata, L., Sanz, D., & Razquín, P. (2016). Performance of Eclipse Farm Test Coupled to e-Reader for Antibiotic Residues Detection in Raw Milk DOI:10.1007/s12161-015-0210-y; Corpus ID: 55682048 Published 1 February 2016 in Chemistry, Food Analytical Methods

McKellar, Q. A., Sanchez Bruni, S. F., & Jones, D. G. (2004). Pharmacokinetic/pharmacodynamic relationships of

antimicrobial drugs used in veterinary medicine. Journal of veterinary pharmacology and therapeutics, 27(6), 503-514.

Navrátilová P., Vyhnálková, J., Zachovalová H., & Vorlová L. (n.d). ACTA VET. BRNO 2020, 89: 201–208; A

microbiological multi-plate method to detect cephalosporin residues in milk, ; https://doi.org/10.2754/avb202089020201

Schermerhorn P.G., Chu, P.-S., & Maureen, A. N. (1998). Determination of Cephapirin

and Ceftiofur Residues in Bovine Milk by Liquid Chromatography with Ultraviolet Detection JOURNAL OF AOAC INTERNATIONAL VOL. 81, No. 5, 1998

Zonca, A., Gallo, M., Locatelli, C., Carli, S., Moroni, P., Villa, R., & Cagnardi, P. (2011). Cefquinome sulfate

behavior after intramammary administration in healthy and infected cows. Journal of Dairy Science, 94(7)

Downloads

Published

2022-05-30

How to Cite

Ilievski, D., Trajkovska, B., & Kochoski, L. (2022). USE OF SCREENING TESTS FOR CEFTIOFUR IN EVALUATION OF RAW MILK SAFETY. KNOWLEDGE - International Journal , 52(3), 381–384. Retrieved from https://ikm.mk/ojs/index.php/kij/article/view/5201