MICROORGANISMS AS OBJECTS FOR EXAMINATION GENOTOXICITY WITH THE USE OF AMES TEST

Authors

  • Ana Coneva Faculty of medical sciences, University Goce Delcev – Stip, Republic of North Macedonia
  • Nevenka Velickova Faculty of medical sciences, University Goce Delcev – Stip, Republic of North Macedonia
  • Vaso Taleski Faculty of medical sciences, University Goce Delcev – Stip, Republic of North Macedonia

Keywords:

microorganism, Ames test, genotoxicity, Salmonella typhimurium

Abstract

Ames test or analysis of reversible mutation of Salmonella typhimurium is a test for identification or detection (screening) of substances that cause genotoxicity or have a mutagenic effect on bacterial cells as a final object. It is a generally accepted test by the WHO used to confirm the genotoxicity of various chemical and physical agents, drugs, additives, preservatives used in various technological or industrial branches. The Ames test is one of the most commonly used tests in toxicology, genetics, microbiology, histology and other branches or scientific disciplines. The Ames test is named after the researcher or person who first applied it in practice, Bruce N. Ames, of the University of California. The purpose of this paper to emphasize the importance and significance of the Ames test and the application of microorganisms as objects for confirmation of genotoxicity in the body. It provides a historical overview of the development and improvement of the Ames test, its advantages and disadvantages, explains in detail the procedure and performance of the test, explains in stages all the changes in Salmonella typhimurium that are the result of certain genotoxicity in the body, compares the Ames test with other in vivo and in vitro genotoxicity tests and a review of several studies that used the same test was evaluated.

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Published

2022-03-30

How to Cite

Coneva, A., Velickova, N., & Taleski, V. (2022). MICROORGANISMS AS OBJECTS FOR EXAMINATION GENOTOXICITY WITH THE USE OF AMES TEST. KNOWLEDGE - International Journal , 51(4), 621–626. Retrieved from https://ikm.mk/ojs/index.php/kij/article/view/5105

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