INVESTIGATION OF ANTIMICROBIAL ACTIVITY OF GEUM URBANUM TINCTURE
Keywords:Geum urbanum, phytotherapy, tannins
The task of modern phytotherapy is to find promising medicinal plants in order to create new herbal medicines. Obviously, herbal medicinal products, to some extent, can be considered as an alternative to antibiotics, while some forms of bacterial infections caused by multiresistant strains of gram positive and gram negative bacteria resistant to other classes of antimicrobial agents and chemicals used to treat a variety of dosage forms. Among the biologically active substances /BAS/ that accumulate in plants and show pharmacological activity, tannins occupy an important place. Such plants include Geum urbanum L. The study of antimicrobial activity of the phytopreparation - tinctures of rhizomes and roots of Geum urbanum L. is an urgent task in terms of prospects for use in medical practice. The goal is to study the effect of tincture of rhizomes and roots of Geum urbanum L. on antimicrobial activity against museum strains of Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica serovar abony. The object of the study was a tincture made from subterranean organs - rhizomes and roots of Geum urbanum L., collected during fruiting in the vicinity of the city of Burgas (Bulgaria). Obtaining tincture was carried out by maceration extraction according to European Pharmacopoeia. To study the antimicrobial activity undiluted tincture and tincture diluted with water in a ratio of tincture-water 1:4, 1:8 and 1:16 were used. Museum strains of microorganisms, as well as standard nutrient media, on which the wells were applied tinctures in certain dilutions. A solvent of 40% ethanol was added to the control wells. The antimicrobial activity of the objects was evaluated by measuring the diameters of the growth retardation zones in Petri dishes. The study of tinctures for antimicrobial activity was performed by diffusion into agar by suppression in the cultivation of bacteria on meat-peptone agar (MPA), followed by incubation in a thermostat at 37o C for 24 hours. Results of obtaining tincture of rhizomes and roots of Geum urbanum L. inhibits the growth of both gram-positive - Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, and gram-negative bacteria - Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica serovar abony. The best results in inhibiting the growth of both gram-positive and gram-negative bacteria were recorded in undiluted tincture obtained from Geum urbanum L.. Tincture of rhizomes and roots of Geum urbanum L. has antimicrobial activity and can be used in medical practice for the treatment of diseases caused by these microorganisms.