KNOWLEDGE OF ANALYTICAL PRINCIPLES IN LABORATORY HEMATOLOGY AND THEIR RELATION TO THE MOST COMMON ERRORS IN THE PREANALYTICAL STAGE
Keywords:
analytical principles, stages, resultsAbstract
Analytical principles in the clinical laboratory have been adopted by the International Federation of
Clinical Chemistry and Laboratory Medicine, the Institute for Clinical and Laboratory Standards, the International
Committee for Standardization. They are described and approved as an appendix to the Medical Standard "Clinical
Laboratory" by regulation of the Minister of Health.
The main goal of laboratory tests is to obtain reliable results, which are a real assessment of the true values and
bring useful information about the patients state of health. The reliability of a laboratory result is a function of a
large number of factors that, by different mechanisms, influence the final result. These factors are grouped in several
directions, one of them includes before the analytical stage in which they fall - taking, storing and transporting the
biological material.
One of the most common mistakes when obtaining the biological material for hematological studies is clotted blood.
Blood for blood count or hematological tests is taken with monovet with anticoagulant EDTA, keeping a certain
blood-anticoagulant ratio, well mixed. The biological material thus obtained is examined with the help of a
hematological analyzer or a counter for blood pictures- the concentration of hemoglobin, the number of blood cells
in a volume of blood, etc. is examined. The analytical principles of these analyzers imply the use of properly taken
biological material, clotted blood gives inaccurate results, there is also a risk of blocking the analyzer.
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