CATALASE ACTIVITY IN RAT BLOOD SERUM UNDER THE IMPACT OF IONIZED WATER SUPPLEMENTED WITH GLUTATHIONE AND VITAMIN C DURING HYPERTHERMIC STRESS
Keywords:
catalase, ionized water, hyperthermic stress, glutathione, vitamin CAbstract
Heat stress is thought to be a factor in the environment that increases the production of reactive oxygen
species (ROS) due to the similarities between the responses that follow heat stress and those that occur during
oxidative stress. Alkaline water, commonly referred to as ionized or reduced water (ERW), is water that has been
electrochemically activated and has a pH higher than 7. Otherwise, the ERW possesses exceptional redox
characteristics and has an alkaline pH and many reducing features. Our study's objective was to examine the effects
of ERW on catalase activity under hyperthermic stress by including enzymatic and non-enzymatic antioxidants,
glutathione, and vitamin C. White laboratory Wistar rats of the female sex, weighing 180–220 g, young rats,
separated into three groups of 15, were used in the experiment. Acute hyperthermic exposure at 41°C led to the
development of oxidative stress. The first group, known as the control group (CPM), is given tap water treatment,
the second group, ionized water treatment (TAM), and the third group, ionized water treatment with additional
glutathione and vitamin C (TAD). The treatment lasted for 21 days. All three groups, concerning the respective
treatment that each of them individually received and the time of its application, do not show a statistically
significant difference in CAT activity, in the period 7-14 days, when the rats were not exposed to high ambient
temperature. Acute hyperthermic exposure caused a significant increase (p < 0.001) in CAT activity in all three
groups. From the statistical analysis of the compared groups on the 7th day of treatment, it is observed that there is
no significant difference in the enzyme mentioned above activity. This finding is identical to the comparison made
concerning the CPM and TAM groups on the 14th and 21st days of the experiment, which is contrary to the
difference in CAT activity between the remaining groups compared during those days, which was shown to be
statistically significant. Treatment with ERW, without added antioxidants or with their combination, did not lead to
significant changes in the activity of CAT in the blood serum during the absence of high ambient temperature.
Moderate levels of free radicals induce increased expression of genes for the synthesis of antioxidant enzymes as a
compensatory mechanism to better protect against ROS-induced damage. This data explains the higher activity of
CAT in the period of hyperthermic stress in the blood serum
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