• Aleksandra Silovska Nikolova Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
  • Daniela Belichovska Institute of Animal Science Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia


nitrites, nitrates, substitute, antimicrobial effect, safety


Ever since ancient times man has sought to increase the sustainability and safety of excess meat and fish. The first methods of conservation were developed based on experience, and later they were developed based on scientific facts. Today, modern production of processed meat can hardly be imagined without the use of preservatives. The most commonly used preservatives in the meat industry are the nitrites: potassium nitrite (E 249) and sodium nitrite (E 250),and the nitrates: sodium nitrate (E 251) and potassium nitrate (E252). Their use limits and prevents the growth and multiplication of microorganisms. At the same time, it also inhibits the growth and multiplication of the pathogenic bacterium Clostridium botulinum. In addition to their preserving effect, nitrites are used in the meat processing industry for the purpose of development and stabilization of the rosy-red color in meat products, prevention of fat oxidation, as well as improving the taste of the finished product. Nitrates, unlike nitrites, do not have a significant impact on the growth and multiplication of microorganisms and the development of other technological benefits. Therefore, they need to be reduced to nitrites with the help of nitrate - reducing bacteria. When nitrites and nitrates are added during the meat processing, nitrite reacts with the amines and amides from the meat. Consequently, carcinogenic and toxic compounds, the N - nitroso compounds are formed. Today, modern consumers are starting to take more care of their health and change their eating habits. They reduce the consumption of processed meat or want to consume naturally processed meat that contains a smaller amount of additives. During the past few decades, much work has been done on the partial or complete replacement of nitrites with alternative natural sources of nitrites in the production of processed meat.


Alahakoon, A.U., Jayasena, D.D., & Ramachandra, S., Jo C. (2015). Alternatives to nitrite in processed meat: Up to date. Trends in Food Sci and Tech., 45(1): 37-49.

Antonescu, V. (1982). Physicochemical study of flavonoids from Origanum vulgare. Farmacia (Bukarest) 30:201-208.

Archer, D. L. (2002). Evidence that ingested nitrate and nitrite are beneficial to health. Journal of Food Protection 65(5): 872-875.

Aureli, P. (1992). Antimicrobial activity Safety 6, 24–29. of some plant essential oils against Listeria monocytogenes. Journal Ting. J. Food. Prot. 55:334-348.

Bassi, D., Puglisi, E., & Cocconcelli, P. S. (2015). Comparing natural and selected starter cultures in meat and cheese fermentations. Curr. Opin. Food Sci. 2 118–122.

Choi, S.H., & Chin, K.B. (2003). Evaluation of sodium lactate as a replacement for conventional chemical preservatives in comminuted sausages inoculated with Listeria monocytogenes. Meat science, 65, 531-537.

Daferera, D. J. (2000). GC-MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. J. Agric. Food Chem. 48(6):2576-2581.

Danker, J., Tromp, T.F., de Vries, H., & Klasen, H.J. (1979). Antimicrobial activity of crude juices of Allium ascalonicum, Allium cepa and Allium sativum. Zentralbl. Bakteriol. (orig A) 245 (1-2):229-239.

De Mey, E., De Maere, H., Paelinck, H., & Fraeye, I. (2017). Volatile N-nitrosamines in meat products: Potential precursors, influence of processing, and mitigation strategies. Food Science and Nutrition. 57 (13): 2909-2923.

Domokos, J. (1997). Essential Oil of Rosemary (Rosmarinus officinalis L.) of Hungarian Origin J. Ess. Oil Res. 9(1):41-45.

Eisinaite, V., Vinauskiene, R., Viskelis, P., & Leskauskaite, D. (2016). Effects of Freeze-Dried Vegetable Products on the Technological Process and the Quality of Dry Fermented Sausages. Journal of Food Sciences 81(9):C2175-82.

Eskandari, M. H., Hosseinpour, S., Mesbahi, G., & Shekarforoush, S. (2013). New composite nitrite-free and low-nitrite meat-curing system using natural colorants. Food Science & Nutrition. 1(5): 392-401.

Gassara, F., Kouassi, A. P., Brar, S. K., & Belkacemi, K. (2016). Green Alternatives to Nitrates and Nitrites in Meat-based Products- A Review. Food Science and Nutrition. 56:13, 2133-2148.

Gill, A.O., & Holley, R.A. (2003). Interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, nisin and EDTA in the presence of nitrite and sodium chloride at 24 oC. Int J Food Microbiol 80:251–259.

Goswami, M., Prabhakaran P.P., & Tanwar, V.K. (2014). Antioxidant and antimicrobial effects of condiments paste used as nitrite replacer in chicken mince. Veterinary World, 7(6): 432-438. Hernandez‐Ramirez, R.U., M.V. Galvan‐Portillo, M.H. Ward, A. Agudo, C.A. Gonzalez.

Ha, J., Gwak, E., Oh, M.H., Park, B., Lee, J., Kim, S., Lee, H., Lee, S., Yoon, Y., & Choi, K.H. (2016). Kinetic behavior of Salmonella on low NaNO2 sausages during aerobic and vacuum storage. Korean J Food Sci An; 36:262-6.

Hammes, P.W. (2012). Metabolism of nitrate in fermented meats: The characteristic feature of a specific group of fermented foods. Food Microbiol 29:151-156.

Hansel, R., Sticher, O., & Steingger, E. (1999). Pharmakognoise, Phytopharmazie,6. Aulf., Springer Verlag, Berlin, Heidelberg, New York

Haraguchi, H., Saito, T., Ishikawa, H., Date, H., Kataoka, S., Tamura, Y., & Mizutani, K. (1996). Antiperoxidative components in Thymus vulgaris. Planta Med. Jun; 62(3):217-21.

Hotchiss, J.H., & Cassens, R.G. (1987). Nitrate, nitrite and nitrozo compounds in foods. Food Technology, 41, 4, 127-134.

Incze, K. (2002). Fermented meat products - A review of current research topics. Fleischwirtschaft, 82, 112-118.

Incze, K. (2003). Ungariche Elesicheugnisse von hoher Qualitӓt und der EU-Beitritt. Mitteilungsblat BAFF, 42, 160, 79-85.

Kovačević, D., Mastanjević, K., Ćosić, K., & Pleadin, J. (2016). Količina nitrita i nitrata u mesnim proizvodima s hrvatskog tržišta. Meso. 18(2): 40-46.

Lagan, J.D., Seman, D.L., Milkowski, A.L., Hirschey, J.A., & Vandeven, M.H. (2004). Modeling the growth boundary of Listeria monocytogenes in ready-to-eat cooked meat products as a function of the product salt, moisture, potassium lactate, and sodium diacetate concentrations, J Food Prot 67:2195–2204.

Laranjo, M., Elias M., & Fraqueza M. J. (2017). The use of starter cultures in traditional meat products. J. Food Qual. 2017:9546026.

Laranjo, M., Potes, M.E., & Elias, M. (2019). Role of Starter Cultures on the Safety of Fermented Meat Products. Front. Microbiol. 10:853.

Lee, S., Lee, H., Kim, S., Lee, J., Ha, J., Choi, Y., Oh H., Choi K.H., & Yoon, Y. (2018). Microbiological safety of processed meat products formulated with low nitrite concentration - A review. Asian-Australasian journal of animal sciences, 31(8), 1073–1077. doi:10.5713/ajas.17.0675.

Madentzidou, E., Gerasopoulos, D., Simos, A., & Bloukas, I. (2012). Salt-stressed fresh cut leek accelerates CO₂ and C₂H₄ production and enhances the development of quality characteristics of traditional Greek sausages during storage. Meat Science, 92(4):789-794.

Maksimović, Ž.A., Hulak, N., Vuko, M., Kovačević, V., Kos, I., & Mrkonjić, M. (2015). Lactic acid bacteria in traditional dry sausage production. Meso: Prvi hrvatski časopis o mesu, 6, 575-580.

Maksimović, Ž.A., Hulak, N., Vuko, M., Kovačević, V., Kos, I., & Mrkonjić, M. (2015). Lactic acid bacteria in traditional dry sausage production. Meso: Prvi hrvatski časopis o mesu, 6, 575-580.

Martinović, A., & Vesković Moračanin, S. (2006). Primena starter kultura u industriji mesa. Tehnologija mesa, 47, 5-6, 216-230.

O'Boyle, A. R., Aladin-Kassam, N., Rubin, L. J., & Diosady, L. L. (1992). Encapsulated cured- meat pigment and its application in nitrite - free ham. Journal of Food Science, 57, 4: 807-812

Paul, S.K., Samanta, G., Halder, G., & Biswas, P. (2007). Effect of a combination of organic acid salts as antibiotic replacer on the performance and gut health of broiler chickens. Livestock Research for rural development 19, 1-8.

Pavlinić Prokurica, I., Bevardi, M., Marušić, N., Vidaček, S., Kolarić Kravar, S., & Medić H. (2010). Nitriti i nitrati kao prekursori N-nitrozamina u paštetama u konzervi. Meso. 12(6): 322-330.

Pichner, R., Hechelmann, H., Steinrueck, H., & Gareis., M. (2006). Shigatoxinproducing Escherichia coli (STEC) in conventionally and organically produced salami products. Fleischwirt. 86(10): 112–114.

Pierson, M. D., & Smoot, L. A. (1982). Nitrite, nitrite alternatives and the control of Clostridium botulinum in cured meats. Critical Reviews in Food Science and Nutrition, 17, 141–187.

Pradhan, A. K., Ivanek, R., Grohn, Y. T., Geornaras, I., Sofos, J. N., & Wiedmann, M. (2009). Quantitative risk assessment for Listeria monocytogenes in selected categories of deli meats: impact of lactate and diacetate on listeriosis cases and deaths. Journal of Food Protection, 72(12), 978e989.

Rački, Đ., Galić, K., Delaš, F., Klapec, T., Kipčić, D., Katalenić, M., Dimitrov, N., & Šarkanj, B. (2010). Kemijske i fizikalne opasnosti u hrani, Hrvatska agencija za hranu (HAH), Osjek, Hrvatska.

Sebranek, J. G. (1979). Advances in the technology of nitrite use and consideration of alternatives. Food Technology, 33(7), 58–62, 93.

Sebranek, J. G., & Bacus, J. N. (2007a). Cured meat products without direct addition of nitrate or nitrite: what are the issues?. Meat Science. (77), 136-147.

Sebranek, J., & Bacus, J. N. (2007b). Natural and Organic Cured Meat Products: Regulatory, Manufacturing, Marketing, Quality and Safety Issues. American Meat Science Association. 1: 1-15.

Seman, D.L, Borger, A.C, Meyer, J.D, Hall, P.A., & Milkowski, A.L. (2002). Modeling the growth of Listeria monocytogenes in cured ready-toeat processed meat products by manipulation of sodium chloride, sodium diacetate, potassium lactate, and product moisture content. J Food Prot 65:651-658.

Shahidi, F., & Pegg, R.B. (1992). Nitrite-free meat curing systems: Update and review. Food Chemistry 43, 185-191.

Silovska Nikolova, A., & Belichovska, D. (2020a). Application of nitrites and nitrates as preservatives in processed meat production. Knowledge - International Journal, 38(3), 525–530.

Silovska Nikolova, A., & Belichovska, D. (2020). Determination of the used additives and their correct declaration, in accordance with the legislation for meat products. knowledge - international journal, 42(3), 481–486.

Silva, C. C. G., Silva, S. P. M., Ribeiro, S. C. (2018). Application of Bacteriocins and Protective Cultures in Dairy Food Preservation. Frontiers in Microbiology. 9(594): 1-15.

Sindelar, J. J., & Milkowski, A. L. (2011). Sodium Nitrite in Processed Meat and Poultry Meats: A Review of Curing and Examining the Risk/Benefit of Its Use. American Meat Science Association. 3: 1-14.

Tompkin, R.B. (2005). Nitrite. In: Antimicrobials in Food. P. Michael Davidson, John n. Sofos, A. L. Branen. Taylor and Francis group, pp. 169-236.

Weber, H. (2004). What supstances do and how they do it. Fleischwirtschaft International, 4, 28-31.

Weiss, J., Gibis, М., Schuh, V., & Salminen, H. (2010). Advances in ingredient and processing systems for meat and meat products. Meat Sci 86:196-213.

Wirth, F. (1991). Restricting and dispensing with curing agents in meat products. Fleischwirtschaft, 71, 9: 1051-1054

Данев, М. (1999). Хигиена и технологија на месо, риби, јајца и нивни производи. Ник Микена – Битола.

Пејковски, З. (2000). Можности за супституција на нитритите во барени колбаси. Докторска дисертација. Земјоделски факултет, Скопје.




How to Cite

Silovska Nikolova, A., & Belichovska, D. (2022). POSSIBILITIES OF NITRITE REPLACEMENT IN MEAT PRODUCTS. KNOWLEDGE - International Journal , 51(3), 465–470. Retrieved from https://ikm.mk/ojs/index.php/kij/article/view/5086

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