THE EFFECT OF CIA ON SERUM IgM VALUES IN LABORATORY WISTAR RATS OF BOTH SEXES

Abstract

Scientific knowledge still cannot specify the etiology of rheumatoid diseases, and the professional literature only mentions some of the risk factors. According to a number of authors, RA occurs as a result of a complex process of immune auto-aggression where the body's defense system recognizes certain transformed proteins in it as foreign and creates antibodies against them, initiating a rather complex process of immune auto-aggression and the creation of immune complexes . The process results in inflammation of the inner wall of the joints, during which the synovium hypertrophies, joint changes and destruction appear, vasculitis develops, subcutaneous nodules, skeletal muscle changes, weakened motor strength and possible changes to the heart, kidneys, lungs and nervous system. As an experimental model, we used two-month-old male and female white Wistar rats, kept in laboratory conditions with food and water ad libitum. The animals were divided into four groups; two controls with 20 male and female individuals and two groups with 30 treated male and female animals each. We applied the collagen prepared according to a defined protocol in the right back joint. 100 μl of collagen solution with 20 μl of physiological solution was applied. We took the blood for analysis from the tail of all individuals, and at the end of the research after sacrificing the animals. After coagulation of the blood, the test tubes were centrifuged, and the serum was decanted into monovets for analysis. The determination of IgM was performed using column chromatography and according to a prescribed operating procedure with Dimension RL Max, an apparatus from the Siemens product line. The immunoglobulin content at the end of the procedure is obtained according to the absorbance and the total volume of the eluate. The CIA values obtained for the male group show that there are changes in the level of serum IgM in animals that were treated compared to untreated, but these are without statistical significance. The values in the female experimental group show that CIA does not have an inhibitory effect on this immunoglobulin in the serum, and on the thirtieth day of treatment there is also an increase in the level of IgM, although this value is not statistically significant. Some authors state that even more than 80% of patients have a decrease in IgA and IgG, but an increase in IgM. Our obtained results coincide with these data and show a suppressive effect of RA on IgA and IgG, but not on the level of the serum value for IgM, where increased values without statistical significance are recorded. The conclusion of the research is that CIA has a teratogenic effect on treated male and female animals with an increase in IgM values, which are without statistical significance.