THE EFFECT OF CIA ON SERUM IgA LEVELS IN WISTAR RATS
Keywords:
RA, CIA, IgA, immune system, ratAbstract
Rheumatoid arthritis is a chronic, evolving inflammatory disease with joint destruction and systemic manifestations, which begins gradually, and usually first affects the metatarsophalangeal and radiocarpal joints. The body's immune system creates antibodies to defend against bacteria, viruses and other microorganisms, while in autoimmune diseases the immune system reacts with its antibodies against the cells of its own tissue. The etiology of RA has a genetic and epigenetic history. It is assumed that some genetic factors, infectious diseases, and mostly viral infections are the reason for the appearance of the disease. It has been proven that it is about activating the patient's immune system in such a way that the own cells are recognized as foreign, which results in an autoimmune response. The process begins when lymphocytes as immune cells are activated and produce cytokines as chemical messengers. The subject of our interest in this study was to experimentally monitor the level of IgA after RA induced by collagen type-II in two experimental periods of treatment, at one and two months, compared to the control groups of both sexes of experimental animals. For this purpose, we used eight-week-old Wistar rats, raised in standard conditions at a temperature of 20°C, with food and water - ad libitum. They were divided into four groups; group of control males, control females, treated males and treated females. We applied the prepared collagen according to a standard protocol and purified form in an amount of 100 μl collagen solution and 20 μl physiological solution in the right hind joint of each treated animal. The examination of the IgA level was performed in both periods after immunization by the method of column chromatography on the Dimension RL Max device from the manufacturer Siemens, according to a defined work procedure. We finally calculated the content of immunoglobulins according to the obtained absorbance at 280 nm and the total volume of the eluate. The obtained values for IgA show that regardless of gender in the treated experimental groups there are no significant statistical changes for this parameter, while, for example, in the treated male group on the thirtieth and on the sixtieth day of the experiment CIA has almost no effect on the level of IgA in comparison with the control group of male animals. A result that stands out is the increase in the level of IgA on the 60th day of treatment in the female arthrosis group, compared to the control group by 30%. The obtained results lead to the conclusion that CIA type-II has the effect of a statistically insignificant decrease in the level of IgA in the serum at the end of the experimental period, i.e. on the 60th day in arthritic male animals compared to the control, while in the same period in female animals non-significant increase in IgA value compared to the control group.
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